Welcome to the world of chromatography! What is chromatography, simply, it is a broad range of physical methods used to separate and analyze complex mixtures. The components to be separated are distributed between two phases: a stationary phase and a mobile phase which percolates through the stationary bed.
Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. The analyte is the substance to be separated during chromatography. It is also normally what is needed from the mixture.
Chromatography is based on the concept of adsorbtion. Solute partitions between two immiscible solvents. When we make one solvent immobile (stationery phase) and another mobile it results in most common applications of chromatography. If support is polar, it is normal phase, and if it is non-polar (C-18) it is reverse phase chromatography.
For chromatography to work effectively, we need the components of mobile phase to separate out as much as possible as they move past the stationary phase. That's why the stationary phase is often something with a large surface area, such as filter paper, some other highly adsorbent material.
Chromatography may be preparative or analytical. The purpose of preparative chromatography is to separate the components of a mixture for more advanced use (and is thus a form of purification). Analytical chromatography is done normally with smaller amounts of material and is for measuring the relative proportions of analytes in a mixture. In other words, preparative chromatography is used to purify sufficient quantities of a substance(such as organic-impurities in pharma analysis) for further use, rather than analysis. Analytical chromatography is used to determine the existence and possibly also the concentration of analyte(s) in a sample.
What is Chromatography used for?
Many branches of science use chromatography to improve our quality of life. Whatever scientific career you choose, you may encounter the use of chromatography.
Drug testing-Many companies require applicants to undergo drug testing, and laboratories often use a chromatograph for that purpose.
Perfume production—During developing new perfumes and during production of existing perfumes, scientists can monitor the composition of samples to ensure consistent quality.
Pollution monitoring—Environmental agencies often use liquid chromatography to monitor air and water, determining the composition and checking for dangerous pollutants, such as lead.
International Journal of Pharmacy and Technology
, Volume Number:
4(1) , Page Number:
Digital Object Identifier: https://dx.doi.org/10.6084/m9.figshare.1008041.v1
Bavirisetty Venkata Kiran Says
goody On 2017-07-30 09:5
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