Chromatography is a method that helps scientists, law enforcement and companies determine the composition of a particular sample. Basically, scientists need a method to separate organic from inorganic compounds. The word itself means "color writing" and the method was developed in the beginning of twentieth century. Since the 1950's, chromatography has promptly developed as an important tool for analyzing materials and samples of unknown mixtures. Chromatography is a great tool that we have developed to answer crucial questions involving health and public safety. Through the use of controlled adsorption chromatography helps us get to the bottom of things.
How does chromatography work?
Chromatography can be compared to a running race. Waiting on the starting line, you've got a mixture of chemicals in some unidentified liquid or gas, comparable to load of runners all mixed up together. When a race starts, runners soon spread out because they have different strengths. In exactly the same way, chemicals in something like a moving liquid mixture spread out because they travel at different speeds over a stationary solid. The most important thing to remember is that chromatography is surface phenomenon based on partition coefficient.
Chromatographic techniques by physical state of mobile phase
Gas chromatography (GC) is a technique in which the mobile phase is a gas. Separation is always carried out in column, which is typically "packed" or "capillary. Capillary columns give superior resolution compared to "packed" columns. Both types of column are made from non-adsorbent and chemically inert materials.
Liquid chromatography (LC) is a technique in which the mobile phase is liquid. It can be carried out either in a column or a plane. Present day liquid chromatography that generally utilizes small packing particles and high pressure is referred to as HPLC.
What is Chromatography used for?
Let’s take a look at some examples of scientific uses for this versatile tool that can be used for very small samples.
Food industry—The food industry uses thin layer chromatography to detect pesticides or insecticides and other food contamination.
Drug production—Pharmaceutical companies use chromatography to help develop new drugs as well as to monitor the purity and consistency of existing products
Fish and wildlife Agencies use chromatography to determine whether there are PCBs (polychlorinated biphenyls) in fish.
International Journal of Pharmacy and Pharmaceutical Sciences
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4(5) , Page Number:
Digital Object Identifier: https://dx.doi.org/10.6084/m9.figshare.1008042.v1
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