What actually is chromatography? The two parts of the word define it quite well. “Chromo(a)-“ indicates color“ -graphy” means writing.
Chromatography provides techniques for separating complex mixtures of liquids or gases, organic or inorganic substances, into their component parts. That’s accomplished by observing at what rates they are absorbed in different substances, such as a strip of paper (paper chromatography) or a column of silica (column chromatography).
Chromatography has been around for longer than a century. A Russian botanist, Mikhail Tswett, developed the process just after the turn of the 20th century to study plant pigments such as chlorophyll.
How does Chromatography work?
No matter the type of chromatography we’re considering, all depend on components moving through a substance, which can be called differential migration. At the conclusion of the process, we measure the differences in the distances and rates that substances move. The process involves two stages: the mobile stage and the stationary stage. The results come from measuring the point at which different compounds stop moving. At that point, they have separated from other compounds.
What types of chromatography are there?
Paper chromatography: It is a technique involving capillary action which moves the solvent through the stationary phase.
Thin layer chromatography (TLC): is a widely employed laboratory technique, similar to paper chromatography. Instead of using a stationary phase of paper, it involves a stationary phase, thin layer of adsorbent like cellulose on a flat, inert substrate.
Column chromatography: Column chromatography is a separation technique in which the stationary bed is within a tube. Differences in rates of movement of analytes through column are different retention times of the sample.
Many branches of science use chromatography to improve our quality of life.
Chromatography separates and analyzes drugs, vitamins, preservatives and proteins in foods to determine nutritional content (quality control in our pharmacy)..
Chromatography plays important role in food regulation; thin layer chromatography is used to detect pesticides or insecticides and other food contamination.
Environmental agencies often use liquid chromatography to monitor air and water, quality and checking for dangerous pollutants, such as lead, mercury..etc.
Journal Name: International Journal of Pharmaceutical Sciences Review and Research , Volume Number: 4(7) , Page Number: 2782Download
Login to Write ReviewLogin