A rapid, specific and accurate isocratic HPLC method was developed and validated for the assay of abacavir sulfate in pharmaceutical dosage forms. The assay involved an isocratic – elution of abacavir sulfate in Grace C18 column using mobile phase composition consists of (38:62 v/v) of methanol and 10ml of potassium dihydrogen orthophosphate. The wavelength of detection is 255nm.The method showed good linearity in the range of 10-50.0mg/mL. The runtime of the method is 8 mins. The proposed method can be used for routine quality control samples in industry in bulk and in finished dosage forms. In present study, a rapid specific precise and validated HPLC method for the quantitative estimation of abacavir sulfate in pharmaceutical dosage forms has been reported. The developed method can be applied to directly and easily to the analysis of the pharmaceutical
Abacavir sulfate[(1R)-4-[2-(amino-6-cyclopropylamino0-9H-purine-9-yl-2-cyclo-pentene]-1-methanol.(Fig-1).Antiretroviral drugs like nucleoside reverse transcriptase inhibitors, non nucleoside reverse transcriptase inhibitors, and protease inhibitors are essential in the management of HIV infection. Abacavir, chemically known as [(1R)-4-[2-amino-6-(cyclopropylamino)-9H-purin-9- yl}-2-cyclopentene]-1-methanol, is a carbocyclic synthetic analogue. Its active metabolite carbovir triphosphate, an analogue of deoxyguanosine-5’-triphosphate (d GTP), inhibits the activity of HIV-1 reverse transcriptase both by competing with the natural substrate dGTP and by its incorporation into viral DNA. Abacavir sulphate is the most powerful nucleoside analog reverse transcriptase inhibitor (NART) used to treat HIV and AIDS. Abacavir is a carbocyclic synthetic nucleoside analogue. Intracellularly, abacavir is converted by cellular enzymes to the active metabolite, carbovir triphosphate (Indian Pharmacopoeia, 1996).
, Digital Object Identifier: https://doi.org/10.6084/m9.figshare.1008040.v1